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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
Ǹgụ́ụ̀bọ̀chị̀
Mkpọlọ́gwụ̀ Orü ọnwé
Odé ákwụ́kwọ́ Thomas Shafee
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I, the copyright holder of this work, hereby publish it under the following license:
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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  • i nye – ikọpị,ikekasi na izịpụ ọru a
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Ọ ga bụ na ọnọdụ ndi a:
  • í-kpó-áhà – Ị ga-enyerịrị ugo kwesịrị ekwesị, nye njikọ na ikikere ahụ, ma gosikwa ma emere mgbanwe. Ị nwere ike ime ya n'ụzọ ezi uche ọ bụla, mana ọ bụghị n'ụzọ ọ bụla na-egosi na onye nyere ikikere kwadoro gị maọbụ ojiji gị.
  1. Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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dị ùgbu â12:20, 13 Febụwarị 2017NvóÁká màkà otù ȯ dị nà 12:20, 13 Febụwarị 20171,020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
12:19, 13 Febụwarị 2017NvóÁká màkà otù ȯ dị nà 12:19, 13 Febụwarị 20171,020 × 803 (90 KB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 Febụwarị 2017NvóÁká màkà otù ȯ dị nà 10:48, 13 Febụwarị 20171,020 × 803 (88 KB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
08:41, 10 Febụwarị 2017NvóÁká màkà otù ȯ dị nà 08:41, 10 Febụwarị 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription
05:08, 5 Ọktoba 2016NvóÁká màkà otù ȯ dị nà 05:08, 5 Ọktoba 20161,020 × 803 (85 KB)Evolution and evolvabilityUser created page with UploadWizard

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