Usòrò:Summary of SAGE.svg

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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1]
Ǹgụ́ụ̀bọ̀chị̀
Mkpọlọ́gwụ̀ Orü ọnwé
Odé ákwụ́kwọ́ Thomas Shafee
Nke ya ozor

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

Nkwényé

I, the copyright holder of this work, hereby publish it under the following license:
w:en:Creative Commons
í-kpó-áhà
This file is licensed under the Creative Commons Attribution 4.0 International license.
I wepulara nóru:
  • i nye – ikọpị,ikekasi na izịpụ ọru a
  • i dowaria – igbanwee ọrụ a
Ọ ga bụ na ọnọdụ ndi a:
  • í-kpó-áhà – Ị ga-enyerịrị ugo kwesịrị ekwesị, nye njikọ na ikikere ahụ, ma gosikwa ma emere mgbanwe. Ị nwere ike ime ya n'ụzọ ezi uche ọ bụla, mana ọ bụghị n'ụzọ ọ bụla na-egosi na onye nyere ikikere kwadoro gị maọbụ ojiji gị.
  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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depicts Bekee

Serial analysis of gene expression Bekee

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